Continuous Fluorescent Diubiquitin Substrates: A New Substrate Discovery Platform

Contact Boston Biochem to begin the substrate optimization for your DUB

• DUB substrate specificity is influenced by many parameters and variables.
• These include but are not limited to: isopeptide linkages, fluor labels, tags and label/tag positions.
• Due to these variables, there is no universal diubiquitin substrate for all DUBs.
• Variables must be optimized for each DUB.

Over 50 unique diubiquitin building blocks are available to create optimal substrates for your DUB. Choose from any available:

• Linkage position- Isopeptide bonds available at K48, K63 and K11 positions
• Fluor labels- For FRET, quench pairs, fluorescent polarization and direct detection
• Affinity tags- For secondary detection reagent or capture assay formats
• Label/tag positions- Fluor labels and tags located in multiple, specific positions allow for >50 distal and anchor ubiquitin combinations
• Ubiquitin mutations- Incorporation of lysine and alternative amino acid mutations

Since 2008, Boston Biochem has successfully developed custom diubiquitin substrates and sourced high throughput screens for the following assay formats:

• Direct or Internal FRET
• DELFIA
• FRET using secondary detection reagents
• ALPHA Screen
• IQP (Internal Quench Pairs)
• Various capture assays