Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin

An efficient linear solid-phase peptide synthesis of ubiquitin (Ub) has been developed. This approach allows the incorporation of desired tags and mutations (see picture; blue denotes a pseudoproline dipeptide, red a dimethoxybenzyl dipeptide) as well as specific C-terminal modification and the construction of all diubiquitin conjugates in high yields and purities in a straightforward manner.