Ubiquitin - DUB-Resistant, Non-Hydrolyzable Chains

Unanchored (free c-terminus) polyubiquitin chains have been identified as potent and essential components in signaling pathways with functions just now starting to be discovered. The identification of pathways and cellular processes that utilize free polyubiquitin chains are difficult to identify due to their transient nature. To facilitate research in the area of unanchored polyubiquitin chains, we have developed a series of non-hydrolyzable polyubiquitin chain reagents. We offer chains of various linkages (linear, K11, K48, and K63) and lengths (di-, tri-, tetra, etc.) with two types of DUB-resistant linkages (NH and DCA). These chains are available free or covalently coupled to agarose for affinity pull downs.

• DUB resistant chains
• Various linkages
• UBA/UIM binding studies
• Signal transduction studies
• Stable in crude lysates
• Free chains
• Agarose bound for affinity applications

NH, Isopeptide-linked Chains - These are chains that contain bona fide isopeptide bonds. An amino acid modification on the C-terminus of ubiquitin makes these chains DUB-resistant.

DCA-linked Chains - Dichloroacetone (DCA) forms of di-ubiquitin (Ub2) are chemically linked via a DCA linker using cysteine introduced into the ubiquitin sequence at the appropriate residues to mimic a native isopeptide linkage. DCA linkage is stable and cannot be processed by deubiquitinases.

NH, Isopeptide-linked chains