E1 Activating

Ub and UBL-dependent metabolism is a multi-enzyme process involving the successive activities of distinct conjugating enzymes. The E1 activating enzymes are central to ubiquitin or ubiquitin-like modifier (UBL) conjugation in vivo and in vitro since they initiate the cascade of conjugation by activating their respective modifiers. Each activating enzyme works in a parallel, but distinct way. The E1 for UBL NEDD8 will not activate SUMO, for example. Each enzyme catalyzes the reaction of ATP and Ub or UBL to activate the C-terminus of the modifier with a high energy thiolester bond. It is this high energy bond that is then transferred to the E2 carrier enzyme for the modifier for the next step in conjugation.


Enzymes

E-300

GST-Ubiquitin E1 Enzyme (UBE1)

Recombinant Yeast

50 µg

$205 Data Sheet Buy / Info

E-304

His6-Ubiquitin E1 Enzyme (UBE1)

human recombinant

50 µg

$175 Data Sheet Buy / Info

E-305

Ubiquitin Activating Enzyme (UBE1)

human recombinant

25 µg

$249 Data Sheet Buy / Info

E-306

GST-Ubiquitin E1 Enzyme (UBE1)

human recombinant

50 µg

$215 Data Sheet Buy / Info

E-307

GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6)

human recombinant

25 µg

$275 Data Sheet Buy / Info

E-308

GST-ISG15 E1/UBE1L

human recombinant

50 µg

$305 Data Sheet Buy / Info

E-309

ISG15 E1/UBE1L

human recombinant

25 µg

$275 Data Sheet Buy / Info

E-310

GST-SUMO E1 (SAE1/UBA2)

S. cerevisiae

50 µg

$295 Data Sheet Buy / Info

E-311

SUMO E1 (SAE1/UBA2)

S. cerevisiae

25 µg

$269 Data Sheet Buy / Info

E-312

GST-NEDD8 E1 (APPBP1/UBA3)

human recombinant

50 µg

$295 Data Sheet Buy / Info

E-313

NEDD8 E1 (APPBP1/UBA3)

human recombinant

25 µg

$265 Data Sheet Buy / Info

E-315

SUMO E1 (SAE1/UBA2)

human recombinant

25 µg

$219 Data Sheet Buy / Info

E-318

New

His6-ATG7 isoform 1

Human Recombinant

50 µg

$299 Data Sheet Buy / Info

E-319

His6-UFM1 Activating Enzyme (UBA5)

human recombinant

25 µg

$269 Data Sheet Buy / Info
Inhibitors

I-502

Nedd8 - E1 Enzyme (NAE Inhibitor)

Synthetic

1 mg

$119 Data Sheet Buy / Info