Ubiquitin

U-590

Ubiquitin modified with fluorescein via primary amine coupling. This results in multiple fluoresceinated ubiquitin species with modified lysines as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to efficiently incorporate into poly-ubiquitin chains.

Ubiquitin Fluorescein

$139.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffer or DMSO up to 20 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Fluorescein-ubiquitin gives a strong signal in the range of 0.1-1 µM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 

Storage: 
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256:9235-9241

UK Price: 
116
Euro Price: 
150
CHF Price: 
186
SKK Price: 
1433
DKK Price: 
1143
NOK Price: 
1377

U-580

Produced via a proprietary process resulting in a single fluorescein modification exclusively on the N-terminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. This reagent allows for poly-ubiquitin chain incorporation of fluorescein-N-terminal ubiquitin with higher efficiency and detection sensitivity than traditionally modified ubiquitins.

Ubiquitin N-Terminal Fluorescein

$159.00
Product Source: 
Human recombinant
Information
Stock: 

Reconstitute in aqueous buffer or DMSO at 1 mg/ml

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

lyophilized powder

Use & Storage
Use: 

Fluorescein-N-term-ubiquitin gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions.  Optimal fluorescence at pH 8.0 is monitored using excitation and emission wavelengths of 490nm and 515 nm, respectively. 

Storage: 

Store at -20°C once reconstituted.  Avoid multiple freeze/ thaw cycles.

Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256:9235-9241

UK Price: 
132
Euro Price: 
170
CHF Price: 
217
SKK Price: 
1612
DKK Price: 
1298
NOK Price: 
1554
Place on New product page: 
Do not list on product page

UB-HK480

Ideal as an alternative to radio-labeled ubiquitin, with detection by avidin-linked reagents. Allows for poly-ubiquitin chain incorporation of biotin-N-terminal ubiquitin with higher efficiency and detection sensitivity than traditionally modified biotin-ubiquitin. Produced via a proprietary process resulting in a single biotin modification exclusively on the N-terminus of ubiquitin. This ubiquitin mutant contains only a single lysine (K48) with all other lysines mutated to arginine, and is able to form poly-ubiquitin chains with other ubiquitin molecules via the K48 lysine only.

His6-Ubiquitin w/K48 only N-Term Biotin

$159.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 20 mg/ml.

MW: 
9.3 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature
References: 

Chau V., et al. (1989) Science 243:1576-1583

Corsi D., et al. (1995) J. Biol. Chem. 270 (15):8928-35.

Gregori L., et al. (1990) J. Biol. Chem. 265:8354-8357

Haldeman M.T., et al. (1997) Biochemistry 36:10526

Mitsui A.,et al. (1999) PNAS 96:6054-6059

Pickart C.M., et al. (1997) FASEB J. 11:1055-1066

Varadan R., et al. (2004) J. Biol. Chem. 279:7055-7063

UK Price: 
132
Euro Price: 
170
CHF Price: 
217
SKK Price: 
1643
DKK Price: 
1293
NOK Price: 
1554

UB-HK630

Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin.

His6-Ubiquitin w/K63 only N-Term Biotin

$159.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 20 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

UK Price: 
132
Euro Price: 
170
CHF Price: 
217
SKK Price: 
1643
DKK Price: 
1293
NOK Price: 
1554

UB-K630

Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin.

Ubiquitin w/K63 only N-Term Biotin

$215.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 20 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

UK Price: 
171
Euro Price: 
222
CHF Price: 
284
SKK Price: 
2142
DKK Price: 
1721
NOK Price: 
2048

UB-K480

Produced via a proprietary process resulting in a single biotin modification exclusively on the N- terminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for poly- ubiquitin chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin.

Ubiquitin w/K48 only N-Term Biotin

$215.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 20 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

UK Price: 
171
Euro Price: 
222
CHF Price: 
284
SKK Price: 
2142
DKK Price: 
1721
NOK Price: 
2048

UB-NOK

Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus. This ubiquitin mutant lacks all reactive lysine residues which have been mutated to arginine. These mutations render the protein unable to form poly-ubiquitin chains and can be used as a negative control or to detect mono-ubiquitination. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with antiubiquitin antibodies.

Ubiquitin No Lys,N-Terminal Biotin

$159.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 5 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

UK Price: 
132
Euro Price: 
170
CHF Price: 
217
SKK Price: 
1643
DKK Price: 
1293
NOK Price: 
1554
Place on New product page: 
Do not list on product page

UB-560

Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin.

Ubiquitin N-Terminal Biotin

$199.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 20 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

UK Price: 
158
Euro Price: 
200
CHF Price: 
216
SKK Price: 
1978
DKK Price: 
1488
NOK Price: 
1810

U-570

Ubiquitin modified with biotin via primary amine coupling. This results in multiple biotinylated ubiquitin species modified at the N-terminus, as well as lysine residues. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate polyubiquitin chains. Biotinylated ubiquitin can be detected using avidin-linked reagents.

Ubiquitin Biotin

$145.00
Product Source: 
human recombinant
Information
Stock: 

Soluble in aqueous buffers up to 50 mg/ml.

MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Depending on desired assay signal and conditions, biotin-Ub should be used in conjunction with native ubiquitin with a 1:1 to 1:5 ratio of biotin-Ub: native Ub, respectively, with a total combined concentration range of 100-500 μM. Typical amounts for a 20 μL reaction: 5 μg - 15 μg biotin-Ub, 20 μg native ubiquitin. Assessment of ubiquitination performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature
References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

UK Price: 
114
Euro Price: 
130
CHF Price: 
141
SKK Price: 
1286
DKK Price: 
968
NOK Price: 
1177
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