Ubiquitin

UC-15

Linkage specific poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules.  Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

Tetra-Ubiquitin/Ub4 WT Chains (K6-linked)

$249.00
Product Source: 
human recombinant
Information
Stock: 

1 mg/ml (29 µM) in sterile, deionized water

MW: 
34 kDa
Purity: 
>95% by SDS-PAGE, Ub-AQUA analysis provided for each lot
Use & Storage
Storage: 

Store at -20°C.  Avoid multiple freeze-thaw cycles.

UK Price: 
205
Euro Price: 
273
CHF Price: 
330
SKK Price: 
2565
DKK Price: 
2035
NOK Price: 
2457
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U-102

Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress.  PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function.  In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Miofusins.  Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recru

Phospho-Ubiquitin (S65)

$245.00
Product Source: 
Human Recombinant
Information
Stock: 

X mg/ml (X µM) in 10 mM HEPES pH 7.5

MW: 
8.65 kDa
Purity: 
> 98% by SDS-PAGE, > 95% by AQUA analysis
Western Blot Data: 
Use & Storage
Use: 

Reaction conditions will need to be optimized for each specific application. 

Storage: 

Store at -20°C.  Avoid multiple freeze/thaw cycles.

UK Price: 
187
Euro Price: 
258
CHF Price: 
320
SKK Price: 
2475
DKK Price: 
1957
NOK Price: 
2313
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Do not list on product page

A-714

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. Unlike ubiquitination which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site.
Human Pan-SUMO, MAb
$229.00
Product Source: 
Monoclonal Rat
Information
Stock: 
0.5 mg/mL in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide
Isotype: 
IgG2A. Clone # 852721
Western Blot Data: 
Antigen: 
Purified, recombinant human SUMO2. Accession Number P61956
Use & Storage
Use: 
Recommended concentration for Western Blot is 0.1 - 0.5 µg/ml.
Storage: 
Store at -20°C.
Specificity: 
This antibody detects endogenous, human SUMOylated proteins in Western blots, and has similar sensitivity when tested against purified, recombinant SUMO1, SUMO2, SUMO3 and SUMO4.
Literature
References: 

Desterro J.M., et al. (1997) FEBs. Lett. 417: 297-300

Okama T., et al. (1999) Biochem. Biophys. Res. Comm. 254: 693-698

Seeler J-S. and Dejean A. (2003) Nat. Rev. 4: 690-699

Su H-L., et al. (2002) Gene 296: 65-73

Tatham M.H., et al. (2001) J. Biol. Chem. 276: 35368-35374

Yeh E.T.H., et al. (2000) Gene 248: 1-14

UK Price: 
181
Euro Price: 
235
CHF Price: 
315
SKK Price: 
2323
DKK Price: 
1802
NOK Price: 
2171
Place on New product page: 
Do not list on product page
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