Proteasome

E-367

The 19S Proteasome, also known as PA700, is a multi-subunit regulatory particle that associates with the 20S Proteasome core particle to form the 26S Proteasome. The 19S Proteasome can cap the 20S Proteasome at one or both ends and regulate substrate access to proteolytic activity in an ATP-dependent manner by modulating the conformation of the 20S Proteasome and facilitating substrate access to the 20S Proteasome catalytic core (1-3). The 19S Proteasome recognizes ubiquitinated proteins as well as non-ubiquitinated, misfolded proteins.

19S Proteasome, FLAG-UCHL5 (C88A)

$325.00
Product Source: 
Human Recombinant
Information
Stock: 

X mg/ml (X μM) in 20 mM Hepes pH 7.5, 20 mM NaCl, 1 mM DTT, 20% glycerol.

MW: 
700 kDa
Purity: 
This product contains <2% 20S Proteasome contamination based on an in vitro activity assay using the 20S Proteasome substrate, Suc-LLVY-AMC (S-280). No detectable UCHL5 activity present by HA-Ubiquitin Vinyl Sulfone and SDS-PAGE Western Blot analysis.
Use & Storage
Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.

Literature
References: 
  1. Adams, G.M. et al. (1998)  Biochemistry  37:12927.
  2. Chu-Ping, M. et al. (1994)   J. Biol. Chem.  269:3539.
  3. Kohler, A. et al. (2001)   Mol. Cell  7:1143.
  4. Strickland, E. et al. (2000)   J. Biol. Chem.  275:5565.
  5. Finley, D. (2009)   Annu. Rev. Biochem.  78:477.
  6. Stadtmueller, B.M. & C.P. Hill (2011)   Mol. Cell  41:8.
  7. Xie, Y. (2010)   J. Mol. Cell Biol.  2:30.
  8. Lee, B.H. et al. (2010)   Nature  467:179.
UK Price: 
248
Euro Price: 
330
CHF Price: 
474
SKK Price: 
3150
DKK Price: 
2596
NOK Price: 
3020
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