Inhibitor

UL-703

Also known as: HA-SUMO1-VS

This N-terminal HA-tagged SUMO is a potent, irreversible and specific inhibitor of SUMO-specific proteases (SENPs). This protein inhibits the hydrolysis of poly-SUMO chains on substrate proteins in vitro and thus enhances poly-SUMO chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of SENP activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

HA-SUMO1 Vinyl Sulfone

$269.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (XμM) in 50 mM Hepes pH 7.0, 150 mMNaCl, 10 % glycerol. Concentration varies with Lot #.

MW: 
11.1 kDa
Purity: 
> 90%
Use & Storage
Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 μM. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291
Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95
Kessler B.M. (2006) Exp.Rev.Proteomics. 3 :213-221
Love K.R, et al. (2007) Nat.Chem.Biol. 3:697-705
Wang G., et al. (2003) Org.Lett. 5: 737-740

UK Price: 
209
Euro Price: 
284
CHF Price: 
387
SKK Price: 
2646
DKK Price: 
2241
NOK Price: 
2525

UL-702

Also known as: SUMO-VS

A potent and highly specific inhibitor of SUMO-specific isopeptidases (SENPs). This protein blocks the hydrolysis of poly-SUMO chains on substrate proteins in vitro and thus enhances poly- SUMO chain accumulation.

SUMO1 Vinyl Sulfone

$269.00
Product Source: 
human recombinant
Information
MW: 
11.1 kDa
Purity: 
> 98% by HPLC
Solubility: 
X mg/ml (X μM) in 50 mM Hepes pH 7.0, 150mM sodium chloride, 10% glycerol. Actual concentration will vary with specific Lot #.
Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 μM. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291
Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95
Wang G., et al. (2003) Org. Lett. 5: 737-740

UK Price: 
209
Euro Price: 
284
CHF Price: 
387
SKK Price: 
2646
DKK Price: 
2241
NOK Price: 
2525

UL-759

Also known as: HA-SUMO2-VS

This N-terminal HA-tagged SUMO is a potent, irreversible and specific inhibitor of SUMO-specific proteases (SENPs). This protein inhibits the hydrolysis of poly-SUMO chains on substrate proteins in vitro and thus enhances poly-SUMO chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of SENP activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

HA-SUMO2 Vinyl Sulfone

$275.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (XμM) in 50 mM MES pH 6.0, 100 mM NaCl, 10% (v/v) Glycerol

MW: 
12 kDa
Purity: 
> 90%
Use & Storage
Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159

Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291

Di Bacco A., et al. (2006) Mol.Cell.Bio. 26: 4489-4498

Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95

Kessler B.M. (2006) Exp.Rev.Proteomics. 3 :213-221

Love K.R, et al. (2007) Nat.Chem.Biol. 3:697-705

Wang G., et al. (2003) Org.Lett. 5: 737-740

UK Price: 
209
Euro Price: 
299
CHF Price: 
387
SKK Price: 
2646
DKK Price: 
2241
NOK Price: 
2578
Place on New product page: 
Do not list on product page

U-202

Also known as: Ub-VS

A potent and highly specific inhibitor of all ubiquitin C-terminal hydrolases. This protein blocks the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

Ubiquitin-Vinyl Sulfone

$269.00
Product Source: 
human recombinant
Information
Stock: 

Supplied as a 2.15 mg/ml (250 µM) solution in 50 mM MES pH 6.0

MW: 
8.6 kDa
Purity: 
> 98 % by HPLC.
Activity: 

Ki = < 40 pM versus UCHL3 

Use & Storage
Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM. 

Storage: 

Store stock solution at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196
Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291
Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95
Misaghi S., et all. (2005) J.Biol.Chem. 280: 1512-1520
Wang G., et al. (2003) Org.Lett. 5: 737-740
Verma R., et al. (2002) Science. 298: 611-615

UK Price: 
223
Euro Price: 
203
CHF Price: 
220
SKK Price: 
2008
DKK Price: 
1511
NOK Price: 
1838

U-212

Also known as: HA-Ub-VS

HA-Ubiquitin Vinyl Sulfone

$235.00
Product Source: 
human recombinant
Information
Stock: 

Supplied as a 2.5 mg/ml (250 µM) solution in 50 mM MES, pH 6.0

MW: 
9.9 kDa
Purity: 
> 95 % by HPLC.
Activity: 

Ki = <40 pM versus UCHL3

Use & Storage
Use: 

Depending on conditions typical concentrations to fully inhibit DUBs in vitro are 1-5 μM.  Also reacts with some E3 Ubiquitin Ligases such as Parkin.

Storage: 

Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159

Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291

Catic A., et al. (2007) PLoS One 2:e679

Dang L.C., et al. (1998) Biochem. 37:1868-1879

Galardy P., et al. (2005) Methods Enz. 399:120-131

Gredmark S., et al. (2007) J.Virol. 81:10300-10309

Hemelaar J., et al. (2004) Mol.Cel.Biol. 24:84-95

Hershko A. and Rose I.A. (1987) Proc.Natl.Acad.Sci. 84:1829-1833

Love K.R., et al. (2007) Nat.Chem.Biol. 3:697-705

Misaghi S., et all. (2005) J.Biol.Chem. 280: 1512-1520

Schieker C., et al. (2005) J.Virol. 79:15582-15585

Wang G., et al. (2003) Org.Lett. 5: 737-740

Verma R., et al. (2002) Science  298: 611-615

UK Price: 
180
Euro Price: 
230
CHF Price: 
249
SKK Price: 
2275
DKK Price: 
1712
NOK Price: 
2082
Place on New product page: 
Do not list on product page

U-211

Also known as: HA-Ubiquitin Aldehyde (HA-Ub-H)

HA-Ubiquitin Aldehyde

$145.00
Product Source: 
human recombinant
Information
MW: 
9.5 kDa
Purity: 
> 95% by HPLC.
Solubility: 
Resuspend in aqueous buffer to 2 mg/ml. Pellet dry compound prior to reconstitution.
Activity: 

Ki = 2.5 nM vs. UCH-L3

Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Add Recombinant Human HA-Ubiquitin Aldehyde to in vitro assays to inhibit Deubiquitinating enzymes.  The HA-tag allows for detection and purification of Deubiquitinating enzymes activity.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human HA-Ubiquitin Aldehyde concentration of 2-5 μM.

Storage: 
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196
Catic A., et al. (2007) PLoS One 2:679
Dang L.C., et al. (1998) Biochem. 37:1868-1879
Galardy P., et al. (2005) Methods Enz. 399:120-131
Gredmark S., et al. (2007) J.Virol. 81:10300-10309
Hemelaar J., et al. (2004) Mol.Cel.Biol. 24:84-95
Hershko A. and Rose I.A. (1987) Proc.Natl.Acad.Sci. 84:1829-1833
Love K.R., et al. (2007) Nat.Chem.Biol. 3:697-705
Melandri F.D., et al. (1996) Biochem. 35:12893-12900
Ovaa H., et al. (2005) Methods Enz. 399:468-478

UK Price: 
120
Euro Price: 
155
CHF Price: 
168
SKK Price: 
1533
DKK Price: 
1154
NOK Price: 
1403
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