E2 Conjugating

E2-615

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT). Human UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively.  Human UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs.

GST-UbcH5a/UBE2D1

$229.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT

MW: 
43 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Use & Storage
Use: 

Reaction conditions will need to be optimized for each specific application. We recommend an initial UBE2D1 concentration of 0.1-1 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

UK Price: 
171
Euro Price: 
248
CHF Price: 
315
SKK Price: 
2218
DKK Price: 
1803
NOK Price: 
2073
Place on New product page: 
Do not list on product page

E2-660

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a member of the Ubiquitin-conjugating (E2) enzyme family.  UBE2N has a predicted molecular weight of 17 kDa. The human enzyme shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively.  UBE2N has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin. UBE2N localizes to both the nucleus and cytoplasm.

His6-Ubc13/UBE2N

$229.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM DTT

MW: 
18 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.   Requires either UBE2V1 (E2-662) or UBE2V2 for in vitro activity.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

UK Price: 
171
Euro Price: 
248
CHF Price: 
315
SKK Price: 
2218
DKK Price: 
1803
NOK Price: 
2073
Place on New product page: 
Do not list on product page
Syndicate content