E2 Conjugating

New

E2-803

Ubiquitin­-conjugating Enzyme H7 (UbcH7), also known as Ubiquitin­-conjugating Enzyme E2L3 (UBE2L3), is a member of the Ubiquitin-­conjugating (E2) enzyme family.  It has a predicted molecular weight of approximately 18 kDa.  The human UbcH7 protein shares 100% amino acid (aa) sequence identity with the mouse and rat orthologs.  UbcH7 has an E2 catalytic core domain that contains an active site cysteine residue and comprises 152 of its 154 aa residues.  UbcH7 is catalytically active with HECT and RBR domain­containing families of Ubiquitin ligases (E3s).  UbcH7 loca

UbcH7/UBE2L3-Ubiquitin Charged

Yes
$365.00
Product Source: 
Human recombinant
Information
Stock: 

X mg/ml (X mM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 1 mM TCEP

MW: 
26 kDa (18 kDa UBE2L3, 8.6 kDa Ubiquitin)
Purity: 
> 95% by SDS-PAGE under non-reducing conditions and visualized by Colloidal Coomassie Blue stain
Use & Storage
Use: 

Pre-charged UBE2L3 may be added directly to in vitro reactions containing E3 Ubiquitin ligases and ligase substrates (if applicable)—no ATP, E1 enzyme, or extra Ubiquitin are required.  Reaction conditions will need to be optimized for each specific application.  Note: Reducing agents including dithiothreitol (DTT) or mercaptoethanol (βME) may cause unintended thiolytic release of Ubiquitin from the complex—care must be taken if these compounds are present in buffers.  We suggest using a thioester friendly reductant such as TCEP if possible.

Storage: 

Store at -80°C.  Avoid multiple freeze-thaw cycles.  Avoid DTT, βME, and other thiol-containing compounds in buffers.

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New

E2-802

Ubiquitin-­conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-­conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin­-conjugating (E2) enzymes.  Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively.  In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins.  For instance, UbcH5c/UBE2D3 is involved in the polyubiqu

UbcH5c/UBE2D3-Ubiquitin Charged

Yes
$365.00
Product Source: 
Human recombinant
Information
Stock: 

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 1 mM TCEP

MW: 
25 kDa (17 kDa UBE2D3, 8.6 kDa Ubiquitin)
Purity: 
> 95% by SDS-PAGE under non-reducing conditions and visualized by Colloidal Coomassie Blue stain
Use & Storage
Use: 

Pre-charged UBE2D3 may be added directly to in vitro reactions containing E3 Ubiquitin ligases and ligase substrates (if applicable)—no ATP, E1 enzyme, or extra Ubiquitin are required.  Reaction conditions will need to be optimized for each specific application.  Note: Reducing agents including dithiothreitol (DTT) or mercaptoethanol (βME) may cause unintended thiolytic release of Ubiquitin from the complex—care must be taken if these compounds are present in buffers.  We suggest using a thioester friendly reductant such as TCEP if possible.

Storage: 

Store at -80°C.  Avoid multiple freeze-thaw cycles.  Avoid DTT, βME, and other thiol-containing compounds in buffers.

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E2-666

Ubiquitin-­conjugating Enzyme E2N (UBE2N), also known as Ubiquitin­-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein.  Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs.  The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63­linked Ubiquitin chains that can either be unanchored or attac

His6 UBE2N/UBE2V2 Complex

$229.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP

MW: 
18 kDa (UBE2N), 17 kDa (UBE2V2)
Purity: 
>95 % by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain.
Use & Storage
Use: 

Recombinant Human His6­UBE2N/UBE2V2 Complex is a member of the Ubiquitin­conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin­activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles. 

UK Price: 
165
Euro Price: 
226
CHF Price: 
346
SKK Price: 
2218
DKK Price: 
1802
NOK Price: 
2073
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Do not list on product page

E2-654

Ubiquitin­conjugating Enzyme H10 (UbcH10), also known as Ubiquitin­conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin­Conjugating  Enzymes (E2) and has a predicted molecular weight of 20 kDa.  Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C.  UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression.  UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11­linked ubiquitination, and promotes target protein degradat

UbcH10/UBE2C

$229.00
Product Source: 
human recombinant
Information
Stock: 

0.50 mg/ml (25 mM) in 50 mM HEPES, pH 7.0, 200 mM NaCl, 1 mM TCEP, and 10% glycerol

MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized with Colloidal Coomassie Blue stain
Use & Storage
Use: 

Recombinant Human UbcH10/UBE2C is a member of the Ubiquitin­conjugating Enzyme (E2) family that receives Ubiquitin from a Ubiquitin­Activating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store at -80°C.  Avoid multiple freeze-thaw cycles. 

UK Price: 
165
Euro Price: 
226
CHF Price: 
346
SKK Price: 
2218
DKK Price: 
1802
NOK Price: 
2073
Place on New product page: 
Do not list on product page

Lonskaya, I. et al. (2013) Ubiquitination Increases Parkin Activity to Promote Autophagic α-Synuclein Clearance. PLoS ONE 8(12): e83914.

PubMed ID: 
recombinant UbcH7
Product(s) Referenced: 
http://dx.doi.org/10.1371/journal.pone.0083914
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