E1 Activating

E-318

ATG7 is a 78 kDa cytosolic ubiquitin­E1­like enzyme that plays a key role in the autophagy pathway of intracellular bulk degradation.  It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8 proteins including GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A, and the subsequent formation of autophagosomes.  ATG7 is required for mitochondrial removal during erythropoiesis (“mitophagy”) and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C­terminus, a region that is required for ATG8 lipidation.

His6-ATG7 isoform 1

No
$299.00
Product Source: 
Human Recombinant
Information
Stock: 

X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol

MW: 
80 kDa (monomer size)
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Ichimura Y., et al. (2000) Nature 408: 488-492

Komatsue M., et al. (2001) J. Biol. Chem. 276: 9846-9854

Mizushima N., et al. (1998) J. Biol. Chem. 273: 33889-33892

Ohsumi Y., et al. (2001) Nat. Rev.  2: 211-216

Reggiori F. and Klionsky D.J. (2002)  Euk. Cell.  1:11-21

Tanida I., et al. (2001) J. Biol. Chem. 276: 1701-1706

Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744

Yu L., et al. (2004) Science 302: 1500-1502

Yuan W., et al. (1999) Mol. Cell. Biol. 10: 1353-1366

UK Price: 
243
Euro Price: 
330
CHF Price: 
474
SKK Price: 
3090
DKK Price: 
2498
NOK Price: 
2848
Place on New product page: 
Do not list on product page
Syndicate content