Enzymes

E-307

Also known as: UBA6

GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6)

$275.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 
148 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Jin J., et al., (2007) Nature 447: 1135-1138.

Pelzer C., et al., (2007) J. Biol. Chem. 282: 23010-23014

UK Price: 
207
Euro Price: 
206
CHF Price: 
223
SKK Price: 
2038
DKK Price: 
1533
NOK Price: 
1865

E-306

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade.

GST-Ubiquitin E1 Enzyme (UBE1)

$219.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 
136 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542
Haas A.L. and Rose I.A. (1982) J. Biol. Chem. 257:1032910337
Haas A.L., et al. (1982) J. Biol. Chem. 257:2543-2548
Handley P.M., et al. (1991) Proc. Natl. Acad. Sci. 88:258-262
Jonnalagadda S., et al. (1988) J. Biol. Chem. 263:5016-5019
Pickart C.M., et al. (1994) J. Biol. Chem. 269:7115-7123
Salvat C., et al. (2000) Eur. J. Biochem. 267:3712-3722
Stephen A.G., et al (1996) J. Biol. Chem. 271:15608-15614
Wilkinson K.D., et al. (1990) Biochem. 29:7373-7380

UK Price: 
180
Euro Price: 
235
CHF Price: 
254
SKK Price: 
2325
DKK Price: 
1749
NOK Price: 
2127

E-319

Also known as: UBA5

His6-UFM1 Activating Enzyme (UBA5)

$269.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with lot number.

MW: 
48 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Dou, T. et al. (2005) Molec. Biol. Rep. 32: 265-271.

Komatsu, M et al. (2004) EMBO J. 23: 1977-1986.

UK Price: 
209
Euro Price: 
284
CHF Price: 
387
SKK Price: 
2702
DKK Price: 
2241
NOK Price: 
2525

E-309

Also known as: UBA7

This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

ISG15 E1/UBE1L

$275.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
112 kDa
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Yuan W., et al. (2001) EMBO. J. 20: 362-371

UK Price: 
209
Euro Price: 
279
CHF Price: 
387
SKK Price: 
2693
DKK Price: 
2241
NOK Price: 
2730
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