Enzymes

E-312

Also know as: GST-NEDD8 Activating Enzyme (APPBP1/Uba3)

GST-NEDD8 E1 (APPBP1/UBA3)

$295.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl. Actual concentration will vary with specific lot number.

MW: 
135 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Lake M.W., et al. (2001) Nature. 414:325-328

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Walden H., et al. (2003) Nature. 422:330-334

UK Price: 
231
Euro Price: 
309
CHF Price: 
423
SKK Price: 
2947
DKK Price: 
2431
NOK Price: 
2755

E-310

Also know as: GST-SUMO Activating Enzyme (Aos1/Uba2)

Conjugation of the ubiquitin-like modifier SUMO requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO-3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.

GST-SUMO E1 (SAE1/UBA2)

$295.00
Product Source: 
S. cerevisiae
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
140 kDa
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Dohmen R.J., et al. (1995) J. Biol. Chem. 270:18099-18109

Gong L., et al. (1999) FEBS Lett. 448:185-189

Johnson E.S., et al. (1997) EMBO J. 16:5509-5519

Okuma T., et al. (1999) Biochem Biophys Res Commun. 254:693698

Tatham M.H., et al. (2001) J. Biol. Chem. 276:35368-35374

UK Price: 
231
Euro Price: 
309
CHF Price: 
423
SKK Price: 
2947
DKK Price: 
2431
NOK Price: 
2755
Place on New product page: 
Do not list on product page

E-313

Also known as: NEDD8 Activating Enzyme (APPBP1/Uba3)

NEDD8 E1 (APPBP1/UBA3)

$265.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl. Actual concentration will vary with specific lot number.

MW: 
109 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Lake M.W., et al. (2001) Nature. 414:325-328

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Walden H., et al. (2003) Nature. 422:330-334

UK Price: 
223
Euro Price: 
200
CHF Price: 
216
SKK Price: 
1978
DKK Price: 
1488
NOK Price: 
1810

E-315

Also known as: SUMO Activating Enzyme (SAE1/SAE2), or UBA2

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/SAE2) and the Ubc9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high- energy thiolester bond with the cysteine residue of SAE2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of Ubc9.

SUMO E1 (SAE1/UBA2)

$225.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM Hepes pH 8, 200 mM NaCl, 1 mM DTT, 10% glycerol. Actual concentration will vary with specific lot number.

MW: 
110 kDa
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Azume Y., et al. (2001) FASEB. J. 15:1825-1827

Desterro JMP.,  et al. (1999) J. Biol. Chem. 274:10618-10624

Okuma T.,  et al (1999) Biochem Biophys Res Commun. 254:693698

Tatham M.H.,  et al. (2001) J. Biol. Chem. 276:35368-35374

UK Price: 
180
Euro Price: 
240
CHF Price: 
260
SKK Price: 
2374
DKK Price: 
1786
NOK Price: 
2172
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