Enzymes

Arena, G. et al. (2013) "Inorganic stressors of ubiquitin." Inorg Chem 52(16): 9567–9573.

PubMed ID: 
23889301
Product(s) Referenced: 
human recombinant Ub activating enzyme [UBE1]

E-318

ATG7 is a 78 kDa cytosolic ubiquitin­E1­like enzyme that plays a key role in the autophagy pathway of intracellular bulk degradation.  It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8 proteins including GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A, and the subsequent formation of autophagosomes.  ATG7 is required for mitochondrial removal during erythropoiesis (“mitophagy”) and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C­terminus, a region that is required for ATG8 lipidation.

His6-ATG7 isoform 1

No
$299.00
Product Source: 
Human Recombinant
Information
Stock: 

X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol

MW: 
80 kDa (monomer size)
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Ichimura Y., et al. (2000) Nature 408: 488-492

Komatsue M., et al. (2001) J. Biol. Chem. 276: 9846-9854

Mizushima N., et al. (1998) J. Biol. Chem. 273: 33889-33892

Ohsumi Y., et al. (2001) Nat. Rev.  2: 211-216

Reggiori F. and Klionsky D.J. (2002)  Euk. Cell.  1:11-21

Tanida I., et al. (2001) J. Biol. Chem. 276: 1701-1706

Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744

Yu L., et al. (2004) Science 302: 1500-1502

Yuan W., et al. (1999) Mol. Cell. Biol. 10: 1353-1366

UK Price: 
243
Euro Price: 
330
CHF Price: 
474
SKK Price: 
3090
DKK Price: 
2498
NOK Price: 
2848
Place on New product page: 
Do not list on product page

E-311

Also known as: SUMO Activating Enzyme (Aos1/Uba2)

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO- 3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.

SUMO E1 (SAE1/UBA2)

$269.00
Product Source: 
S. cerevisiae
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
114 kDa
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Dohmen R.J., et al. (1995) J. Biol. Chem. 270:18099-18109

Gong L., et al. (1999) FEBS Lett. 448:185-189

Johnson E.S., et al. (1997) EMBO J. 16:5509-5519

Okuma T., et al. (1999) Biochem Biophys Res Commun. 254:693698

Tatham M.H., et al. (2001) J. Biol. Chem. 276:35368-35374

UK Price: 
209
Euro Price: 
284
CHF Price: 
387
SKK Price: 
2702
DKK Price: 
2241
NOK Price: 
2525

E-304

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade.

His6-Ubiquitin E1 Enzyme (UBE1)

$179.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP

MW: 
121 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542
Haas A.L. and Rose I.A. (1982) J. Biol. Chem. 257:1032910337
Haas A.L., et al. (1982) J. Biol. Chem. 257:2543-2548
Handley P.M., et al. (1991) Proc. Natl. Acad. Sci. 88:258-262
Jonnalagadda S., et al. (1988) J. Biol. Chem. 263:5016-5019
Pickart C.M., et al. (1994) J. Biol. Chem. 269:7115-7123
Salvat C., et al. (2000) Eur. J. Biochem. 267:3712-3722
Stephen A.G., et al (1996) J. Biol. Chem. 271:15608-15614
Wilkinson K.D., et al. (1990) Biochem. 29:7373-7380

UK Price: 
142
Euro Price: 
148
CHF Price: 
160
SKK Price: 
1464
DKK Price: 
1102
NOK Price: 
1340
Place on New product page: 
Do not list on product page

E-308

Also known as: UBA7

This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. The enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

GST-ISG15 E1/UBE1L

$275.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 
138 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Yuan W., et al. (2001) EMBO. J. 20: 362-371

UK Price: 
225
Euro Price: 
309
CHF Price: 
423
SKK Price: 
2946
DKK Price: 
2431
NOK Price: 
2792

E-307

Also known as: UBA6

GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6)

$275.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 
148 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Jin J., et al., (2007) Nature 447: 1135-1138.

Pelzer C., et al., (2007) J. Biol. Chem. 282: 23010-23014

UK Price: 
207
Euro Price: 
206
CHF Price: 
223
SKK Price: 
2038
DKK Price: 
1533
NOK Price: 
1865

E-306

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade.

GST-Ubiquitin E1 Enzyme (UBE1)

$219.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 
136 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542
Haas A.L. and Rose I.A. (1982) J. Biol. Chem. 257:1032910337
Haas A.L., et al. (1982) J. Biol. Chem. 257:2543-2548
Handley P.M., et al. (1991) Proc. Natl. Acad. Sci. 88:258-262
Jonnalagadda S., et al. (1988) J. Biol. Chem. 263:5016-5019
Pickart C.M., et al. (1994) J. Biol. Chem. 269:7115-7123
Salvat C., et al. (2000) Eur. J. Biochem. 267:3712-3722
Stephen A.G., et al (1996) J. Biol. Chem. 271:15608-15614
Wilkinson K.D., et al. (1990) Biochem. 29:7373-7380

UK Price: 
180
Euro Price: 
235
CHF Price: 
254
SKK Price: 
2325
DKK Price: 
1749
NOK Price: 
2127

E-319

Also known as: UBA5

His6-UFM1 Activating Enzyme (UBA5)

$269.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with lot number.

MW: 
48 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Dou, T. et al. (2005) Molec. Biol. Rep. 32: 265-271.

Komatsu, M et al. (2004) EMBO J. 23: 1977-1986.

UK Price: 
209
Euro Price: 
284
CHF Price: 
387
SKK Price: 
2702
DKK Price: 
2241
NOK Price: 
2525

E-309

Also known as: UBA7

This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

ISG15 E1/UBE1L

$275.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
112 kDa
Purity: 
> 90% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Yuan W., et al. (2001) EMBO. J. 20: 362-371

UK Price: 
209
Euro Price: 
279
CHF Price: 
387
SKK Price: 
2693
DKK Price: 
2241
NOK Price: 
2730

E-305

Also known as: UBA1

Ubiquitin Activating Enzyme (UBE1)

$249.00
Product Source: 
human recombinant
Information
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 
118 kDa
Purity: 
> 95% by SDS-PAGE
Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.
Literature
References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542
Haas A.L. and Rose I.A. (1982) J. Biol. Chem. 257:1032910337
Haas A.L., et al. (1982) J. Biol. Chem. 257:2543-2548
Handley P.M., et al. (1991) Proc. Natl. Acad. Sci. 88:258-262
Jonnalagadda S., et al. (1988) J. Biol. Chem. 263:5016-5019
Pickart C.M., et al. (1994) J. Biol. Chem. 269:7115-7123
Salvat C., et al. (2000) Eur. J. Biochem. 267:3712-3722
Stephen A.G., et al (1996) J. Biol. Chem. 271:15608-15614
Wilkinson K.D., et al. (1990) Biochem. 29:7373-7380

UK Price: 
191
Euro Price: 
260
CHF Price: 
281
SKK Price: 
2572
DKK Price: 
1935
NOK Price: 
2353
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