Enzymes

E-300

GST-Ubiquitin E1 Enzyme (UBE1)

$215.00
Product Source: 
Recombinant Yeast
Information
Stock: 

0.71 mg/ml (5 µM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

MW: 
141 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain.
Use & Storage
Use: 

Reaction conditions will need to be optimized for each specific application.  We recommend an initial GST-Ubiquitin E1 Enzyme concentration of 50-200 nM.

Storage: 

Store at -80°C and avoid multiple freeze-thaw cycles for best performance.

Literature
References: 

Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543

Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329

Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258

Hershko, A. et al. (1983) J. Biol. Chem. 258:8206

Moundry, P. et al. (2012) Cell Cycle 11:1573

Nagai, Y. et al. (1995) J. Cell Sci. 108:2145

Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188

Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319

Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608

UK Price: 
158
Euro Price: 
210
CHF Price: 
227
SKK Price: 
2077
DKK Price: 
1563
NOK Price: 
1901
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