UM-NOK

Material Data Sheet

Ubiquitin Mutant No K

Price: $89.00
Catalog #: UM-NOK

Ubiquitin Mutant No K

Price: 
$89
Quantity: 
1 mg
This ubiquitin mutant contains no lysine residues, with all lysines mutated to arginine. This mutation renders ubiquitin unable to form poly-ubiquitin chains and is useful as a negative control.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hershko A., et al. (1991) J.Biol.Chem. 266:16376-16379
Hershko A., et al. (1995) Biochem.Biophys.Res.Comm. 128:1079-1086