UM-L8A

Material Data Sheet

Ubiquitin Mutant L8A

Price: $85.00
Catalog #: UM-L8A

Ubiquitin Mutant L8A

Price: 
$85
Quantity: 
1 mg

Ubiquitin has a distinct and functionally important hydrophobic patch whose surface is defined by three residues including Leu8, Ile44 and Val70. These residues are solvent accessible in ubiquitin chains and are critical for poly-ubiquitin chain interaction and recognition by the 26S proteasome and subsequent target degradation. These hydrophobic residues interact directly with various factors that bind to Ub and/or mediate Ub-Ub interactions in ubiquitin chains that may influence orientation and recognition. Ubiquitin L8A can form an E1-catalyzed active thioester at the
C-terminus allowing the molecule to be transferred to the lysines of substrate proteins.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Beal R., et al. (1996) Proc.Natl.Acad.Sci.USA. 93:861-866
Beal R., et al. (1998) Biochemistry. 37:2925-2934
Lam Y., et al. (1997) J Biol Chem. 272:28438-28446
Mueller T.D. and Feignon J. (2002) J.Mol.Biol. 319:1243-1255
Sloper-Mould K.E., et al. (2001) J.Biol. Hem. 276:30483-30489
Varadan R., et al. (2002) J.Mol.Biol. 324:637-647