Ubiquitin Mutant K63R
Mutation of lysine 63 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 63 linkages with other Ub molecules. Ub K63R can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K63 linked.
Soluble and stable in aqueous buffers up to 10 mg/ml.
This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
Arnason T., et al. (1994) Mol.Cell.Biol. 14:7876-7883
Spence J., et al. (1995) Mol.Cell.Biol. 15:1265-1273