UM-K48R

Material Data Sheet

Ubiquitin  Mutant K48R

Price: $89.00
Catalog #: UM-K48R

Ubiquitin  Mutant K48R

Price: 
$89
Quantity: 
1 mg
Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. Ub K48R can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K48 linked.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Chau V., et al. (1989) Science 243:1576-1583
Baboshina D.V.,et al. (1996) J.Biol.Chem. 271:2823-2831
Finley D., et al. (1994) Mol. Cell.Biol. 14:5501-5509
Johnson E.S., et al. (1992) EMBO.J 11:497-5055
Johnson E.S., et al. (1995) J.Biol.Chem. 270: 17442-17756