UM-K480

Material Data Sheet

Ubiquitin Mutant with K48 only

Price: $75.00

Catalog #: UM-K480

Ubiquitin Mutant with K48 only

Price:

Quantity:

1 mg

Data Sheet:

UMK480.pdf

This ubiquitin mutant contains only a single lysine, K48, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules exclusively via the K48 lysine.

Product Information

Stock:

Soluble and stable in aqueous buffers up to 10 mg/ml.

MW:

8.5 kDa

Purity:

> 95% by SDS-PAGE

Supplied:

This is a lyophilized powder

Use & Storage

Use:

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage:

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References:

Chau V., et al. (1989) Science 243:1576-1583
Baboshina D.V., et al. (1996) J.Biol.Chem. 271:2823-2831
Finley D., et al. (1994) Mol.Cell. Biol. 14:5501-5509
Johnson E.S., et al (1992) EMBO.J 11:497-5055
Johnson E.S., et al. (1995) J.Biol.Chem. 270: 17442-17756