UM-K33R

Material Data Sheet

Ubiquitin Mutant K33R

Price: $85.00
Catalog #: UM-K33R

Ubiquitin Mutant K33R

Price: 
$85
Quantity: 
1 mg
Mutation of lysine 33 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 33 linkages with other Ub molecules. Ub K33R can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K33 linked.

Product Information

MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Soluble and stable aqueous buffers up to 5 mg/ml.

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugation reactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ott D.E., et al. (2000) Virology 278:111-121
Spence J., et al. (1995) Mol. Cell. Biol. 15:1265-1273