UM-K330

Material Data Sheet

Ubiquitin Mutant with K33 only

Price: $75.00
Catalog #: UM-K330

Ubiquitin Mutant with K33 only

Price: 
75
Quantity: 
1 mg
Data Sheet: 
application/pdf iconUMK330.pdf
This ubiquitin mutant contains only a single lysine, K33, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K33 lysine.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage: 
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ott D.E., et al. (2000) Virology 278:111-121

Spence J., et al. (1995) Mol.Cell.Biol. 15:1265-1273