UM-K29R

Material Data Sheet

Ubiquitin Mutant K29R

Price: $85.00
Catalog #: UM-K29R

Ubiquitin Mutant K29R

Price: 
$85
Quantity: 
1 mg
Mutation of lysine 29 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 29 linkages with other Ub molecules. Ub K29R can form an E1-catalyzed active thioester at theC-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K29 linked.

Product Information

Stock: 
Soluble and stable aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Arnason T., et al. (1994) Mol.Cell.Biol. 14:7876-7883
Spence J., et al. (1995) Mol.Cell.Biol. 15:1265-1273
Russel N.S., et al. (2004) Biochemistry 43:4844-4854