UM-K110

Material Data Sheet

Ubiquitin Mutant with K11 only

Price: $85.00
Catalog #: UM-K110

Ubiquitin Mutant with K11 only

Price: 
$85
Quantity: 
1 mg
This ubiquitin mutant contains only a single lysine, K11, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K11 lysine.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Baboshina D.V., et al. (1996) J.Biol.Chem. 271:2823-2831
Makhatadze G.T., et al. (2003) J. Mol. Biol. 327:1135-1148