UM-HK6R

Material Data Sheet

His6-Ubiquitin Mutant K6R

Price: $75.00
Catalog #: UM-HK6R

His6-Ubiquitin Mutant K6R

Price: 
75
Quantity: 
1 mg
Data Sheet: 
application/pdf iconum-hk6r-his6-ubiquitin-mutant-k6r.pdf
Mutation of lysine 6 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 6 linkages with other Ub molecules. Ub K6R can form an E1-catalyzed active thioester at the Cterminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and for determining if poly-Ub chains are K6 linked.

Product Information

Stock: 
Soluble and stable aqueous buffers up to 5 mg/ml.
MW: 
9.3 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Baboshina D.V., et al. (1996) J.Biol.Chem. 271:2823-2831
Morris J.R., et al. (2004) Hum. Mol.Genet. 13:807-817
Wu-Baer F., et al. (2003) J.Biol.Chem. 278:34743-34746