UM-HK60

Material Data Sheet

His6-Ubiquitin Mutant with K6 only

Price: $75.00
Catalog #: UM-HK60

His6-Ubiquitin Mutant with K6 only

Price: 
75
Quantity: 
1 mg
Data Sheet: 
application/pdf iconUMHK60.pdf
This ubiquitin mutant contains only a single lysine, K6, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K6 lysine.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 5 mg/ml.
MW: 
9.3 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage: 
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Baboshina D.V., et al. (1996) J.Biol.Chem. 271:2823-2831
Morris J.R., et al. (2004) Hum.Mol.Genet. 13:807-817
Wu-Baer F., et al. (2003) J.Biol.Chem. 278:34743-34746