UM-HK480

Material Data Sheet

His6-Ubiquitin Mutant with K48 only

Price: $85.00
Catalog #: UM-HK480

His6-Ubiquitin Mutant with K48 only

Price: 
$85
Quantity: 
1 mg
This ubiquitin mutant contains only a single lysine, K48, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules exclusively via the K48 lysine.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
9.3 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Chau V., et al. (1989) Science 243:1576-1583
Baboshina D.V., et al. (1996) J.Biol.Chem. 271:2823-2831
Finley D., et al. (1994) Mol.Cell. Biol. 14:5501-5509
Johnson E.S., et al (1992) EMBO.J 11:497-5055
Johnson E.S., et al. (1995) J.Biol.Chem. 270: 17442-17756