UM-HK290

Material Data Sheet

His6-Ubiquitin Mutant with K29 only

Price: $85.00
Catalog #: UM-HK290

His6-Ubiquitin Mutant with K29 only

Price: 
$85
Quantity: 
1 mg
This ubiquitin mutant contains only a single lysine, K29, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K29 lysine.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
9.3 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Arnason T., et al. (1994) Mol.Cell.Biol. 14:7876-7883

Spence J., et al. (1995) Mol.Cell.Biol. 15:1265-1273

Russel N.S., et al. (2004) Biochemistry 43:4844-4854