UM-G76A

Material Data Sheet

Ubiquitin Mutant G76A

Price: $55.00
Catalog #: UM-G76A

Ubiquitin Mutant G76A

Price: 
$55
Quantity: 
100 µg
Mature Ub has a highly conserved C-terminal diglycine motif which is crucial for activity and recognition with conjugation and deconjugation enzyme components. The replacement the last glycine residue with alanine results in a Ub that supports E1-Ub thioester formation and downstream conjugation reactions (transfer to E2, E3) but at a rate ~20% compared to wildtype Ub. This mutant however, inhibits deconjugation and prevents the removal of Ub from modified protein substrates by deubiquitinating enzyme (DUBs). Since this Ub becomes irreversibly conjugated to protein, it shifts the equilibrium between the bound and unbound form in the direction of conjugation, at the expense of the free form.

Product Information

Stock: 
Aqueous solutions up to 5 mg/ml
MW: 
8.6 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dayal S. et al. (2009) J.Biol.Chem. 284:5030-5041
Geng F. and Tansey W.P. (2008) Mol.Biol.Cell. 19:3616-3624
Pickart C.M. et al. (1994) J.Biol.Chem. 269:7115-7123
Ravid T. and Hochstrasser M. (2007) Nat.Cell Biol. 9:422-427
Wilkinson K.D. et al. (1995) Biochem. 34:14535-14546