UM-D58A

Material Data Sheet

Ubiquitin Mutant D58A

Price: $85.00
Catalog #: UM-D58A

Ubiquitin Mutant D58A

Price: 
$85
Quantity: 
1 mg

D58 is a residue that has been identified as being important for binding and recognition by proteins that contain ubiquitin binding domains (UBDs), and represents a new hydrophilic interaction surface on ubiquitin. This residue may be crucial for the interaction and recognition of poly-ubiquitin chains by the 26S proteasome and other enzymes involved in ubiquitination. Ub D58A can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Alam S.L and Sundquist W.I. (2006) Nat.Struc.Mol.Biol. 13:186-188
Hicke L., et al. (2005) Nat.Rev.Mol.Cell.Biol. 6:610-621
Penengo L., et al. (2006) Cell. 124:1183-1195
Raiborg C., et al. (2006) Trends.Biochem.Sci. 31:541-544