UM-3KTR

Material Data Sheet

Ubiquitin Mutant K29R/K48R/K63R

Price: $85.00
Catalog #: UM-3KTR

Ubiquitin Mutant K29R/K48R/K63R

Price: 
$85
Quantity: 
1 mg
Mutation of lysines 29, 48 and 63 to arginine renders ubiquitin unable to form poly-ubiquitin chains via the major known poly-ubiquitination sites. The ubiquitin triple mutant can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for studies of alternate mono-ubiquitination or polyubiquitination sites.

Product Information

Stock: 
Soluble and stable in aqueous buffers up to 5 mg/ml.
MW: 
8.5 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

Literature

References: 

Arnason T., Arnason T., et al. (1994) Mol.Cell.Biol. 14:7876-7883
Baboshina D.V., et al. (1996) J.Biol.Chem. 271:2823-2831
Finley D., et al. (1994) Mol.Cell.Biol. 14:5501-5509
Johnson E.S., et al. (1992) EMBO.J 11:497-5055
Johnson E.S., et al. (1995) J.Biol.Chem. 270: 17442-17756
Spence J., et al. (1995) Mol.Cell.Biol. 15:1265-1273