ULN-320

Material Data Sheet

Poly-SUMO3 Hydrolysis-Resistant Chains (3-8)

Price: $195.00
Catalog #: ULN-320

Poly-SUMO3 Hydrolysis-Resistant Chains (3-8)

Price: 
$195
Quantity: 
50 µg

Sentrin protease (SENP)-resistant poly-SUMO3 chains can be used to investigate mechanisms of chain recognition by SUMO-targeted ubiquitin ligases (STUbl's) or other proteins that contain SUMO-interacting domains (SIM's).  These K-11 linked chains are formed enzymatically with recombinant SUMO3 containing a mutation of glutamine 89 to proline, which renders them approximately 500-fold more resistant to disassembly by SENP's than are wild-type chains.  Mono- and di-SUMO3 have been removed from the chain mixture.

Product Information

Stock: 
X mg/ml in 50 mM Hepes pH 8.0, 100 mM NaCl, 1 mM DTT.
Purity: 
>90% by SDS-PAGE

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Store at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bekes, M., et al. (2011) J. Biol. Chem. 286: 10238-10247

Bylebyl G.R., et al. (2003) J. Biol. Chem. 278: 44113-44120

Dohmen R. J. (2004) Biochem. Biophys. Acta. 1695: 113-131

Johnson E. (2004) Ann. Rev. Biochem. 73: 355-382

Tatham M.H., et al. (2001) J. Biol. Chem. 276: 35368-35374