ULM-762

Material Data Sheet

SUMO3 Mutant K11R

Price: $195.00
Catalog #: ULM-762

SUMO3 Mutant K11R

Price: 
$195
Quantity: 
250 µg

Mutation of lysine 11 to arginine renders SUMO-3 unable to form poly-SUMO multimers and is useful to investigate mono-SUMOylation or can be used to reduce poly-SUMO chain formation. Human SUMO-3 contains the VK11TE sequence which allows for the formation of poly-SUMO chains. K11 is the conserved lysine that becomes modified and is the point of attachment for the C-terminal glycine of the preceding SUMO-3. The function of SUMO chains has not yet been fully elucidated.

Product Information

Stock: 
X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW: 
10.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 
Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bencsath K. P., et al. (2002) J. Biol. Chem. 277: 47938–47945

Dohmen R.J., et al. (2004) Biochem. Biophys. Acta. 1695: 114-131

Johnson E. S. and Gupta A. A., (2001) Cell 106: 735–744

Johnson E.S. (2004) Annu. Rev. Biochem. 73: 355-382

Pedrioli G. A., et al. (2006) Nat. Meth.l 3:533-539

Pichler A., et al. (2002) Cell 108: 109-120

Rodriguez M.S., et al. (2001) J.Biol.Chem. 276: 12654-12659

Sampson D.A., et al. (2001) J.Biol.Chem. 276: 21664-21669

Takahashi Y., et all. (2003) J. Biochem. 133:415–422

Tatham M.H., et al. (2001) J.Biol.Chem. 276: 35368-35374.