ULM-712

Material Data Sheet

SUMO1 Mutant K16R

Price: $195.00
Catalog #: ULM-712

SUMO1 Mutant K16R

Price: 
$195
Quantity: 
250 µg

Mutation of lysine 16 to arginine in SUMO-1 is useful for the analysis of poly-SUMO-1 chain formation. Human SUMO-1 does not contain the exact ψΚXE motif consensus sequence found in SUMO-2 and SUMO-3 proteins, but K16 is the putative site for chain formation. SUMO-1 has been shown to form chains in vitro, but the function of SUMO chains has not yet been fully elucidated.

Product Information

Stock: 
X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW: 
11.1 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.

Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bencsath K. P., et al. (2002) J. Biol. Chem. 277: 47938–47945

Dohmen R.J., et al. (2004) Biochem. Biophys. Acta. 1695: 114-131

Johnson E. S. and Gupta A. A., (2001) Cell 106: 735–744

Johnson E.S. (2004) Annu. Rev. Biochem. 73: 355-382

Pedrioli G. A., et al. (2006) Nat. Meth.l 3:533-539

Pichler A., et al. (2002) Cell 108: 109-120

Rodriguez M.S., et al. (2001) J.Biol.Chem. 276: 12654-12659

Sampson D.A., et al. (2001) J.Biol.Chem. 276: 21664-21669

Takahashi Y., et all. (2003) J. Biochem. 133:415–422

Tatham M.H., et al. (2001) J.Biol.Chem. 276: 35368-35374.