Material Data Sheet

SUMO1 Mutant K7R

Price: $195.00
Catalog #: ULM-710

SUMO1 Mutant K7R

250 µg

Human SUMO-1 does not contain the exact ψΚXE consensus sequence found in SUMO-2 and SUMO-3. Within this motif ψ represents a large hydrophobic amino acid (I, L, or V), K is the lysine that becomes modified, X is any residue and E is glutamic acid. Many known SUMO-1 conjugation sites occur within this consensus sequence, but SUMOylation also occurs on lysine residues located within non-consensus regions. SUMO-1 has been shown to form chains in vitro and in vivo but often the linkage is uncharacterized, and the function of SUMO chains has not yet been fully elucidated. SUMO-1 multimerization in vitro has been shown to occur predominantly via lysines K7, K16 and K17. Mutation of lysine 7 to arginine is useful to investigate mono- SUMOylation requirements or to reduce poly-SUMO chain formation.

Product Information

X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
11.1 kDa
> 95% by SDS-PAGE

Use & Storage


Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.

Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.



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