ULC-320

Material Data Sheet

Poly-SUMO3 Wild-type Chains (3-8)

Price: $150.00
Catalog #: ULC-320

Poly-SUMO3 Wild-type Chains (3-8)

Price: 
150
Quantity: 
25 µg
Data Sheet: 
application/pdf iconulc-320-poly-sumo3-wild-type-chains-3-8.pdf
Poly-SUMO-3 chains can be used to investigate mechanisms of chain recognition, binding and hydrolysis by SUMO-specific isopeptidases (SENPs), SUMO-specific E3 ligases or other proteins that contain SUMO-3 binding domains. This product is formed enzymatically with wildtype human recombinant SUMO-3 linked via lysine 11 which is the point of attachment for the Cterminal glycine of the preceding SUMO-3. Mono-SUMO-3 has been removed from the chain mixture.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 1 mM DTT. Concentration will vary with specific Lot #.
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bylebyl G.R., et al. (2003) J. Biol. Chem. 278:44113-44120
Dohmen R. J. (2004) Biochem. Biophys. Acta. 1695:113-131
Johnson E. (2004) Ann. Rev. Biochem. 73:355-382
Tatham M.H., et al. (2001) J. Biol. Chem. 276: 35368-35374