UL-920

Material Data Sheet

FAT10 Agarose

Price: $159.00
Catalog #: UL-920

FAT10 Agarose

Price: 
$159
Quantity: 
500 µL
Data Sheet: 

FAT10 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of FAT10 interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein.

Product Information

Stock: 
0.5 ml resin supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.
MW: 
18.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 

The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1M KCL. Remove cleaning solution by washing resin with 5ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be used as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Bates E.E., et al. (1997) J. Immunol. 27: 2471-2477

Fan W., et al. (1996) Immunogen. 44: 97-103

Hipp M.S., et al. (2004) J. Biol Chem. 279: 16503-16510

Hipp M.S., et al. (2005) Mol. Cell. Biol. 25: 3483-3491

Lie Y.C., et al. (1999) Proc. Natl. Acad. Sci. 96: 4313-4318

Lim C.B., et al. (2006) Cell.Div. 1:20

Lee C.G., et al. (2003) Oncogene. 22: 2592-2603

Raasi S., et al. (2001) J. Biol Chem. 276: 35334-35343

Ross M.J., et al. (2006) J.Am.Soc.Nephrol. 17: 996-1004