UL-913

Material Data Sheet

FAT10 Fluorescein

Price: $175.00
Catalog #: UL-913

FAT10 Fluorescein

Price: 
175
Quantity: 
50 µg
Data Sheet: 
application/pdf iconul-913-fat10-fluorescein.pdf
FAT10 modified with fluorescein via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated FAT10 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
18.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein-FAT10 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bates E.E., et al. (1997) J. Immunol. 27: 2471-2477

Fan W., et al. (1996) Immunogen. 44: 97-103

Hipp M.S., et al. (2004) J. Biol Chem. 279: 16503-16510

Hipp M.S., et al. (2005) Mol. Cell. Biol. 25: 3483-3491

Lie Y.C., et al. (1999) Proc. Natl. Acad. Sci. 96: 4313-4318

Lim C.B., et al. (2006) Cell.Div. 1:20

Lee C.G., et al. (2003) Oncogene. 22: 2592-2603

Raasi S., et al. (2001) J. Biol Chem. 276: 35334-35343

Ross M.J., et al. (2006) J.Am.Soc.Nephrol. 17: 996-1004