UL-912

Material Data Sheet

FAT10 Biotin

Price: $95.00
Catalog #: UL-912

FAT10 Biotin

Price: 
$95
Quantity: 
50 µg
Data Sheet: 

Human Leukocyte Antigen-F Associated Transcript 10 (FAT10), also known as Ubiquitin D (UBD), is a 165 amino acid (aa) member of the Ubiquitin-like family of proteins. Human FAT10 has a predicted molecular weight of 18.5 kDa and shares 69% aa sequence identity with mouse FAT10.  FAT10 mRNA is expressed as a single transcript in lymphoblastoid lines and dendritic cells, and is induced by IFN-gamma and TNF-alpha in some cell lines.  Structurally, FAT10 consists of two Ubiquitin-like domains that are connected by a short linker. Like Ubiquitin, FAT10 has a C-terminal glycine residue that can be used to form isopeptide bonds with target proteins. FAT10-conjugated proteins are targeted to the proteasome where the 26S Proteasome subunit S5a/Angiocidin binds to FAT10 and enables subsequent degradation of the conjugated protein.  In addition to S5a/Angiocidin, FAT10 has been shown to interact with Huntingtin, Ataxin-1, MAD2, and NUB1L. FAT10 has been implicated in a number of biological processes such as cell cycle control, antigen presentation, and cytokine response.

This protein is modified with biotin via primary amine coupling resulting in the modification of lysine residues as well as the N-terminus. 

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
19 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Biotin-FAT10 can be conjugated to substrate proteins via the subsequent actions of a FAT10-activating (E1) enzyme, a FAT10-conjugating (E2) enzyme, and a FAT10 ligase (E3). Biotin-FAT10 is ideal for the visualization or quantification of thioester formation with avidin-linked reagents. Reaction conditions will need to be optimized for each specific application. We recommend an initial Biotin-FAT10 concentration of 5-20 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.