UL-912

Material Data Sheet

FAT10 Biotin

Price: $80.00
Catalog #: UL-912

FAT10 Biotin

Price: 
$80
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-912 biotFAT10_.pdf
Stock: 

X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.

MW: 
18.5 kDa
Purity: 
> 95% by SDS-PAGE
FAT10 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated FAT10 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies.

Use & Storage

Use: 
Depending on experimental conditions and detection method, suggested in vitro concentrations for thiolester formation is 5 to 20 μM.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bates E.E., et al. (1997) J. Immunol. 27: 2471-2477
Fan W., et al. (1996) Immunogen. 44: 97-103
Hipp M.S., et al. (2004) J. Biol Chem. 279: 16503-16510
Hipp M.S., et al. (2005) Mol. Cell. Biol. 25: 3483-3491
Lie Y.C., et al. (1999) Proc. Natl. Acad. Sci. 96: 4313-4318
Lee C.G., et al. (2003) Oncogene. 22: 2592-2603
Raasi S., et al. (2001) J. Biol Chem. 276: 35334-35343