Material Data Sheet
FAT10 is a ubiquitin-like protein discovered by chromosmal sequencing in the human MHC class I locus. The protein consists of two ubiquitin-like domains in a head-to-tail arrangement and contains a C-terminal di-glycine which is critical for covalent conjugation to target proteins. FAT10 expression is inducible by the IFN-γ and TNF-α cytokines, and it may modulate tumorigenesis through its reported interaction with the MAD2 spindle-assembly checkpoint protein. Proteins conjugated to FAT10 are rapidly degraded by the proteasome.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual protein concentration will vary with specific lot number.
Purity:> 95% by SDS-PAGE
Store at -80°C. Avoid multiple freeze/thaw cycles.
Bates E.E., et al. (1997) J. Immunol. 27: 2471-2477
Fan W., et al. (1996) Immunogen. 44: 97-103
Hipp M.S., et al. (2004) J. Biol Chem. 279: 16503-16510
Hipp M.S., et al. (2005) Mol. Cell. Biol. 25: 3483-3491
Lie Y.C., et al. (1999) Proc. Natl. Acad. Sci. 96: 4313-4318
Lee C.G., et al. (2003) Oncogene. 22: 2592-2603
Raasi S., et al. (2001) J. Biol Chem. 276: 35334-35343