Material Data Sheet

NEDD8-13C 15N

Price: $195.00
Catalog #: UL-845

NEDD8-13C 15N

100 µg
Data Sheet: 

Isotopically labeled NEDD8  is useful in determining total cellular concentrations of NEDD8 using the protein standard absolute quantification (PSAQ) or related methods.  Highly purified 13C/15N labeled NEDD8 processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the Ubc12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase.  NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family. 

Product Information

X mg/ml (X µM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 0.5 mM EDTA, 1 mM DTT. Actual concentration will vary with specific Lot #.
9012.5 Da (95% labeled)
> 95% by SDS-PAGE

Use & Storage


Typical amount for use as an internal recovery standard is approximately 1 µg per gram of protein lysate.

Lyophilized powder at 4oC. Solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.



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