UL-845

Material Data Sheet

NEDD8-13C 15N

Price: $195.00
Catalog #: UL-845

NEDD8-13C 15N

Price: 
$195
Quantity: 
100 µg
Data Sheet: 

Isotopically labeled NEDD8  is useful in determining total cellular concentrations of NEDD8 using the protein standard absolute quantification (PSAQ) or related methods.  Highly purified 13C/15N labeled NEDD8 processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the Ubc12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase.  NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family. 

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 0.5 mM EDTA, 1 mM DTT. Actual concentration will vary with specific Lot #.
MW: 
9012.5 Da (95% labeled)
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical amount for use as an internal recovery standard is approximately 1 µg per gram of protein lysate.

Storage: 
Lyophilized powder at 4oC. Solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042

Hori T., et al. (1999) Oncogene.  18:6829-6834

Kaieser S., et al.  (2011) Nature Methods 8: 691-696

Kamura T., et al. (1999) Genes. Dev.  13:2928-2933

Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399

Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398

Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105

Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991