UL-840

Material Data Sheet

NEDD8 Agarose

Price: $159.00
Catalog #: UL-840

NEDD8 Agarose

Price: 
$159
Quantity: 
500 µL
Data Sheet: 

Nedd8 is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of Nedd8 activating E1 enzyme, the Nedd8 carrier enzyme Ubch12, Nedd8 E3 ligases, and other proteins/enzymes that have an affinity for Nedd8. Nedd8 is poly-His6 tagged. The ubiquitin-like protein Nedd8 is conjugated to targets by the Nedd8-specific E1 activating enzyme (AppBp1/Uba3), the Ubch12 E2 enzyme, and the ROC1/Rbx1 RING finger E3 ligase. Nedd8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Product Information

Stock: 
0.5 ml Nedd8-agarose is supplied in a 1 ml total volume of Hepes buffered solution.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 

Nedd8-agarose can be re-used for at least 10-15 applications if properly maintained. After application, clean resin by washing with 10 ml 50 mM Tris-Cl pH 9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing up to 1 mM NaN3.
Resin should be stored at 4°C. DO NOT FREEZE

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991