UL-835

Material Data Sheet

NEDD8 Rhodamine

Price: $175.00
Catalog #: UL-835

NEDD8 Rhodamine

Price: 
$175
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-835 RhoNEDD8_.pdf
Stock: 

X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.

MW: 
12.1 kDa
Purity: 
> 95% by SDS-PAGE

NEDD8 modified with rhodamine red via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-NEDD8 chains. This labeled NEDD8 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies.

Use & Storage

Use: 
Rhodamine NEDD8 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex570 nm and Em590 nm wavelengths respectively. 
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991