UL-830

Material Data Sheet

NEDD8 Fluorescein

Price: $195.00
Catalog #: UL-830

NEDD8 Fluorescein

Price: 
$195
Quantity: 
50 µg
NEDD8 modified with fluorescein via primary amine coupling is ideal as an alternative to radiolabeled NEDD8. This results in multiple fluoresceinated NEDD8 species with modified lysines as well as the N-terminus. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Product Information

Stock: 
X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
MW: 
9 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein-NEDD8 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex494 nm and Em520 nm wavelengths respectively.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991