Material Data Sheet

NEDD8 Fluorescein

Price: $195.00
Catalog #: UL-830

NEDD8 Fluorescein

50 µg
NEDD8 modified with fluorescein via primary amine coupling is ideal as an alternative to radiolabeled NEDD8. This results in multiple fluoresceinated NEDD8 species with modified lysines as well as the N-terminus. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Product Information

X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
9 kDa
> 95% by SDS-PAGE

Use & Storage


Fluorescein-NEDD8 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex494 nm and Em520 nm wavelengths respectively.

Store at -80°C. Avoid multiple freeze/thaw cycles.



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