Material Data Sheet

NEDD8 Biotin

Price: $95.00
Catalog #: UL-820

NEDD8 Biotin

50 µg
Data Sheet: 

NEDD8 modified with biotin via primary amine coupling is ideal as an alternative to radiolabeled NEDD8. This results in multiple biotinylated NEDD8 species with modified lysines as well as the N-terminus. Biotinylated NEDD8 can be detected using avidin-linked reagents. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Product Information

X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
9 kDa
> 95% by SDS-PAGE

Use & Storage


Suggested concentration for in vitro assays is 5-25 μM.

Store at -80°C. Avoid multiple freeze/thaw cycles.



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