UL-815

Material Data Sheet

Methylated NEDD8

Price: $215.00
Catalog #: UL-815

Methylated NEDD8

Price: 
$215
Quantity: 
500 µg

The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the Ubch12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family. Methylated NEDD8 is ideal for determining whether mono- or poly-NEDD8 substrate modification is required for function.

Product Information

MW: 
9 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Soluble and stable in DMSO and aqueous buffers up to 10 mg/ml.
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 

Storage: 
Lyophilized product can be stored at 4oC. Once reconstituted, store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991