UL-812

Material Data Sheet

NEDD8

Price: $150.00
Catalog #: UL-812

NEDD8

Price: 
$150
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL812_Nedd8.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
9 kDa
Purity: 
> 95% by SDS-PAGE

The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Use & Storage

Use: 
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991