UL-810

Material Data Sheet

Pro-NEDD8

Price: $235.00
Catalog #: UL-810

Pro-NEDD8

Price: 
$235
Quantity: 
1 mg
Data Sheet: 

Protein precursor to functional NEDD8 which is ideal for the characterization of NEDD8-specific processing proteases. The ubiquitin-like protein Nedd8 is conjugated to targets by the NEDD8- specific E1 activating enzyme (AppBp1/Uba3) the Ubch12 E2 enzyme, and the ROC1/Rbx1 RING finger ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Product Information

Stock: 
[pro- NEDD8] = X mg/ml in 20 mM HEPES pH 8.0, 50 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW: 
9.1 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. et al. (1999) J. Biol. Chem. 274: 12036-12042
Hori T., et al. (1999) Oncogene. 18:6829-6834
Kamura T., et al. (1999) Genes. Dev. 13:2928-2933
Kumar S., et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
Morimoto M., et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
Whitby F.G., et al. (1998) J. Biol. Chem. 273: 34983-34991