UL-804

Material Data Sheet

NEDD8-Aminoluciferin (AML)

Price: $215.00
Catalog #: UL-804

NEDD8-Aminoluciferin (AML)

Price: 
$215
Quantity: 
50 µg

Substrate for deconjugating enzymes (NEDPs) based on the C-terminal derivative of NEDD8 with
aminoluciferin (AML). Rather than fluorescence as the indicator of NEDP activity, NEDP liberated
luciferin is processed by luciferase to give a luminescence signal.
NEDD8-AML not only produces a stronger signal, but also has an excellent signal to noise ratio
over traditional fluorophores. This makes it possible to rapidly assess the activity of NEDPs that
poorly utilize NEDD8-AMC while using much lower levels of the NEDPs themselves.

Product Information

Stock: 
X mg/ml (X μM) in 50mM HEPES pH 7.5, 100mM NaCl.
MW: 
8.8 kDa
Purity: 
>95%
Activity: 

>100:1 signal to noise ratio with 10 pM NEDP1. Km of NEDP1for NEDD8-AML is 69 nM.

Use & Storage

Use: 

NEDD8-AML gives a strong luminescent signal in the range of 0.1-1 μM, depending on experimental conditions. Optimal luminescence at pH 7.5 is monitored using all wavelengths with a 500 ms integration time.

Storage: 
Store at -80°C. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Reddy G., et al. (2010) JACS. 132:39, 13586-13587
Shinde R., et al. (2006) Biochemistry 45: 11103-11112