Material Data Sheet

FLAG-NEDD8 Vinyl Sulfone

Price: $269.00
Catalog #: UL-803

FLAG-NEDD8 Vinyl Sulfone

25 µg
This fully functional N-terminal FLAG-tagged NEDD8 is a potent, irreversible inhibitor of NEDD8-specific isopeptidases (like NEDP1 or SENP8, Cat # E-800). Useful for inhibiting the hydrolysis of poly-NEDD8 chains on substrate proteins in vitro and thus enhances poly-NEDD8 chain accumulation. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This epitope allows for the sensitive identification or purification of NEDD8 deconjugating activities since it is specifically recognized by anti-FLAG antibodies M1, M2 or M5.

Product Information

X mg/ml (XμM) in 50 mM Hepes pH 7.0, 150 mM sodium acetate, 10 % glycerol. Concentration varies with Lot #.
9.1 kDa
> 90% by HPLC

Use & Storage


Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 µM.

Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.



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