UL-803

Material Data Sheet

FLAG-NEDD8 Vinyl Sulfone

Price: $269.00
Catalog #: UL-803

FLAG-NEDD8 Vinyl Sulfone

Price: 
$269
Quantity: 
25 µg
This fully functional N-terminal FLAG-tagged NEDD8 is a potent, irreversible inhibitor of NEDD8-specific isopeptidases (like NEDP1 or SENP8, Cat # E-800). Useful for inhibiting the hydrolysis of poly-NEDD8 chains on substrate proteins in vitro and thus enhances poly-NEDD8 chain accumulation. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This epitope allows for the sensitive identification or purification of NEDD8 deconjugating activities since it is specifically recognized by anti-FLAG antibodies M1, M2 or M5.

Product Information

Stock: 
X mg/ml (XμM) in 50 mM Hepes pH 7.0, 150 mM sodium acetate, 10 % glycerol. Concentration varies with Lot #.
MW: 
9.1 kDa
Purity: 
> 90% by HPLC

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 µM.

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2002) Chem. Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem. Biochem. 6: 287-291
Catic A., et al. (2007) Plos ONE 4: e381-
Frickel E-M., et al. (2007) Cell. Microbiol. 9:11601-1610
Hemelaar J., et al. (2004) Mol. Cell. Bio. 24: 84-95
Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221
Gan-Erdene T., et al. (2003) J. Biol. Chem. 278: 28892-28900
Mendoza H.M., et al. (2003) J. Biol. Chem. 278: 25637-25643
Wang G., et al. (2003) Org. Lett. 5: 737-740
Wu K., et al. (2003) J. Biol. Chem. 278: 28882-288891