UL-802

Material Data Sheet

NEDD8 Vinyl Sulfone

Price: $250.00
Catalog #: UL-802

NEDD8 Vinyl Sulfone

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL802_Nedd8 VS.pdf
Stock: 

X mg/ml (X mM) in 50 mM Hepes pH 7.2. Actual concentration will vary with specific Lot #.

MW: 
9.1 kDa
Purity: 
> 98 % by HPLC.

Also known as: NEDD8-VS

A potent, irreversible and specific inhibitor of NEDD8-specific isopeptidases (like NEDP1 or SENP8). Useful for inhibiting the hydrolysis of poly-NEDD8 chains on substrate proteins in vitro and thus enhances poly-NEDD8 chain accumulation.

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit NEDPs in vitro are 1- 5 μM. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2002) Chem. Biol. 9: 1149-1159
Borodovsky A.,et al. (2005) Chem. Biochem. 6: 287-291
Hemelaar J., et al. (2004) Mol. Cell. Bio. 24: 84-95
Gan-Erdene T., et al. (2003) J. Biol. Chem. 278: 28892-28900
Mendoza H.M., et al. (2003) J. Biol. Chem. 278: 25637-25643
Wang G., et al. (2003) Org. Lett. 5: 737-740
Wu K., et al. (2003) J. Biol. Chem. 278: 28882-288891